UArizona Compare and Contrast the Various Methodologies for Performing IHC Questions

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2. Below is an image of staining for the HER2 protein in breast tumours from two
patients (A and B), using immunohistochemistry.
a) Describe what is observed in each of the patients and explain what diagnoses
would be made and why this would influence how each patient is treated. (8 marks)
b) Compare and contrast the various methodologies for performing IHC. (17 marks)
3. Compare and contrast the principles of Sanger sequencing and pyrosequencing
for determining the base sequence of DNA, and their use in identifying genetic
variants in patient samples. (25 marks)

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