A new amino acid has been isolated that has been titrated to determine the pKaàof the ionizable groups.

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Amino acid Assignment for Biochemistry Spring 2023
Please write the answers to the questions below based on the data I am giving you.
When you complete this assignment bring it to class on Feb 1st.
A new amino acid has been isolated that has been titrated to determine the pKaàof the
ionizable groups. The following data was obtained:
pKa
? carboxyl
1.9
? amino
9.7
? amino
8.1
? amino
7.3
? carboxyl
3.9
a) Show the complete titration for this amino acid based on the information above
(remember to assign charge to each form)
b) What is the pH when the average net charge of the amino acid is +1.5?
c) What is the pI for this amino acid?
d) What forms of the amino acid are at equilibrium at pH 7.3? (Show the charge for each of
ionizable groups at this pH)

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