Enzyme Catalysis
Student Name
Date
1
Data
Activity 1
Data Table 1
0%
25%
50%
75%
100%
Depth of
H2O2
Solution
Trial 1
Time
Trial 2
Time
Trial 3
Time
Avg.
Time
Rate
(d/t)
1. Based on the information in Data Table 1, explain the relationship between
percent concentration of catalase and reaction rate.
2016 Carolina Biological Supply Company
2
Activity 2
Data Table 2
10 deg.
below
Room temp.
Depth of
H2O2
Solution
Trial 1
Time
Trial 2
Time
Trial 3
Time
Avg.
Time
Rate
(d/t)
2016 Carolina Biological Supply Company
10 deg.
above
20 deg.
above
30 deg.
above
3
Activity 3
Data Table 3
pH
1.3
2.3
3.3
4.3
5.3
Well
Location
B1
B2
B3
B4
B5
Depth of
H2O2
Solution
Trial 1
Trial 2
Trial 3
Avg. time
Rate (d/t)
2016 Carolina Biological Supply Company
4
pH
12.7
11.7
10.7
9.7
8.7
Well
Location
D1
D2
D3
D4
D5
Depth of
H2O2
Solution
Trial 1
Trial 2
Trial 3
Avg. time
Rate (d/t)
Graphs
Create graphs using graphing software.
Graph 1
Insert the graph of Activity 1 data.
1. Using the graph, predict the rate if catalase concentrations are 10%, 60%
and 85%.
2016 Carolina Biological Supply Company
5
Graph 2
Insert the graph of Activity 2 data.
2. Interpret the graph for Activity 2. Is there an optimum temperature for
catalase that makes it the most productive in terms of reaction rate? At what
temperature does the rate decrease? Explain why.
Graph 3
Insert the graph of Activity 3 data.
3. Interpret the graph for Activity 3. Is there an optimum pH for catalase that
makes it the most productive in terms of reaction rate? At what pHàdoes the
rate decrease? Explain why.
2016 Carolina Biological Supply Company

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